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expresshyb hybridization solution  (TaKaRa)


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    TaKaRa expresshyb hybridization solution
    Expresshyb Hybridization Solution, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 3756 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hybridization+solution/ExpressHyb+Hybridization+Solution/pm42833298-162-8-11
    Average 95 stars, based on 3756 article reviews
    expresshyb hybridization solution - by Bioz Stars, 2026-11
    95/100 stars

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    Related Articles

    other:

    Article Title: Viral piracy of host RNA phosphatase DUSP11 by avipoxviruses.
    Article Snippet: MSA of AA sequences was visualized using Jalview (S9 and S10 Figs) [54].

    Membrane:

    Article Title: In situ sequence-specific visualization of single methylated cytosine on tissue sections using ICON probe and rolling-circle amplification
    Article Snippet: One pg of four types of plasmid constructs: (1) vector without methylation, (2) vector + RANK gene promoter without methylation, (3) vector with methylation, and (4) vector + RANK gene promoter with methylation, was spotted onto the Hybond N + nylon membrane (Amersham Biosciences Corp., Piscataway, NJ, USA) and immobilized by UV cross-linking. .. The blotted membrane was prehybridized with prehybridization buffer in a sealed bag at 50 °C in a water bath for 2 h. The prehybridization buffer was replaced with hybridization solution (hybridization buffer containing 100 ng/ml of ICON probe for RANK gene promoter end-labeled with γP 32 -ATP with T4 polynucleotide kinase (Takara, Tokyo, Japan) to a specific activity of 2 × 10 8 cpm/ μg. ..

    Hybridization:

    Article Title: In situ sequence-specific visualization of single methylated cytosine on tissue sections using ICON probe and rolling-circle amplification
    Article Snippet: One pg of four types of plasmid constructs: (1) vector without methylation, (2) vector + RANK gene promoter without methylation, (3) vector with methylation, and (4) vector + RANK gene promoter with methylation, was spotted onto the Hybond N + nylon membrane (Amersham Biosciences Corp., Piscataway, NJ, USA) and immobilized by UV cross-linking. .. The blotted membrane was prehybridized with prehybridization buffer in a sealed bag at 50 °C in a water bath for 2 h. The prehybridization buffer was replaced with hybridization solution (hybridization buffer containing 100 ng/ml of ICON probe for RANK gene promoter end-labeled with γP 32 -ATP with T4 polynucleotide kinase (Takara, Tokyo, Japan) to a specific activity of 2 × 10 8 cpm/ μg. ..

    Article Title: β-amylase and method for utilization and production thereof
    Article Snippet: .. The stringent conditions are commonly known by those skilled in the art, and examples thereof include conditions of hybridization at 68° C. in a commercially available hybridization solution, ExpressHyb Hybridization Solution (Takara Bio Inc.), or conditions of hybridization at about 65° C. in the presence of 0.7 M to 1.0 M NaCl and washing at about 65° C. with a 0.1 to 2×SSC solution (1×SSC solution: 150 mM NaCl, 15 mM sodium citrate, pH 7.0), using DNA immobilized on a filter. ..

    Article Title: Viral piracy of host RNA phosphatase DUSP11 by avipoxviruses
    Article Snippet: .. Crosslinked membranes were prehybridized for ~ 1 hour at 55°C in hybridization solution (Takara). .. In brief, total RNA was harvested from cells using TRIzol reagent (Thermo Fisher) or PIG-B [ , ] and fractionated on 10–12% polyacrylamide gel electrophoresis (PAGE)-urea gel and transferred to Amersham Hybond-N+ membrane (GE Healthcare).

    Article Title: A Pseudomonas aeruginosa small RNA regulates chronic and acute infection
    Article Snippet: Approximately 1 μg of total RNA was separated on a 15% polyacrylamide gel electrophoresis–urea gel and transferred to Hybond-N+ membranes (GE Healthcare). .. The membranes were then crosslinked with an ultraviolet crosslinker (UVP) and probed in hybridization solution (Takara) with the indicated DNA oligonucleotides (listed in Supplementary Table ) that were radiolabelled with [γ- 32 P]ATP (Perkin Elmer) by T4 polynucleotide kinase (New England Biolabs). .. Probed membranes were exposed to a phosphor screen and visualized using a Typhoon Biomolecular Imager (GE Healthcare).

    Article Title: Physiological and molecular evidence of heat acclimation memory: a lesson from thermal responses and ischemic cross-tolerance in the heart
    Article Snippet: The unincorporated 32 P-labeled nucleotides were removed by Nucleo-Spin extraction columns (Clontech). .. The membranes were prehybridized for 1 h at 68°C in a hybridization solution (Clontech) with 0.1 mg/ml sheared salmon testes DNA (Sigma) to block nonspecific binding. ..

    Article Title: Viral piracy of host RNA phosphatase DUSP11 by avipoxviruses
    Article Snippet: .. Crosslinked membranes were prehybridized for ∼1 hour at 55C in hybridization solution (Takara). .. DNA oligos ( ) were radiolabeled with [γ- 32 P] ATP (Perkin Elmer) by T4 polynucleotide kinase (New England Biolabs) for ∼1 hour at 37C.

    Activity Assay:

    Article Title: In situ sequence-specific visualization of single methylated cytosine on tissue sections using ICON probe and rolling-circle amplification
    Article Snippet: One pg of four types of plasmid constructs: (1) vector without methylation, (2) vector + RANK gene promoter without methylation, (3) vector with methylation, and (4) vector + RANK gene promoter with methylation, was spotted onto the Hybond N + nylon membrane (Amersham Biosciences Corp., Piscataway, NJ, USA) and immobilized by UV cross-linking. .. The blotted membrane was prehybridized with prehybridization buffer in a sealed bag at 50 °C in a water bath for 2 h. The prehybridization buffer was replaced with hybridization solution (hybridization buffer containing 100 ng/ml of ICON probe for RANK gene promoter end-labeled with γP 32 -ATP with T4 polynucleotide kinase (Takara, Tokyo, Japan) to a specific activity of 2 × 10 8 cpm/ μg. ..

    Blocking Assay:

    Article Title: Physiological and molecular evidence of heat acclimation memory: a lesson from thermal responses and ischemic cross-tolerance in the heart
    Article Snippet: The unincorporated 32 P-labeled nucleotides were removed by Nucleo-Spin extraction columns (Clontech). .. The membranes were prehybridized for 1 h at 68°C in a hybridization solution (Clontech) with 0.1 mg/ml sheared salmon testes DNA (Sigma) to block nonspecific binding. ..

    Binding Assay:

    Article Title: Physiological and molecular evidence of heat acclimation memory: a lesson from thermal responses and ischemic cross-tolerance in the heart
    Article Snippet: The unincorporated 32 P-labeled nucleotides were removed by Nucleo-Spin extraction columns (Clontech). .. The membranes were prehybridized for 1 h at 68°C in a hybridization solution (Clontech) with 0.1 mg/ml sheared salmon testes DNA (Sigma) to block nonspecific binding. ..

    Nonspecific Binding:

    Article Title: Physiological and molecular evidence of heat acclimation memory: a lesson from thermal responses and ischemic cross-tolerance in the heart
    Article Snippet: The unincorporated 32 P-labeled nucleotides were removed by Nucleo-Spin extraction columns (Clontech). .. The membranes were prehybridized for 1 h at 68°C in a hybridization solution (Clontech) with 0.1 mg/ml sheared salmon testes DNA (Sigma) to block nonspecific binding. ..



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